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THE CYTOGENETIC APPROACHES AT STUDY OF BIRDS POPULATIONS

S. Rushkovsky, K. Afanasieva, V. Bezrukov

Department of General and Molecular Genetics, Taras Shevchenko National University of Kyiv,
64 Volodymyrska Str., Kyiv, 01033, Ukraine.

The karyology of birds is less developed in comparison, for example with mammalian karyology. From 17 existing penguin species only 3 species were karyotyped (humbolt's, ostrich and jackass penguins).
Low number of studied karyotypes of penguin species dependents on the one hand with problems of samples collecting, and to another - with common features of karyotypes of birds.
Birds karyotypes have conservative structure: thay have similar high diploid numbers and two conditional classes of chromosomes: macrochromosomes and microchromosome. The presence of microchromosomes complicates detailed analysis of karyotypes, ascertainment exact diploid numbers. Therefore, investigators frequently are limited to analysis macrochromosomes, which have similar morphology (especially first three couples) for the majority of the representatives of the class. Diploid number of chromosome of studyed species of penguins varies in range 70-80, first three couples of macrochromosomes have typical morphology for the majority of birds species.
It is not present sufficient data about a structural of macrochromosomes and quantitative polymorphism of microchromosomes. For study of polymorphism it is necessary to analyze karyotypes enough plenty individuals, but majority of the explorers are limited to one or two animals. Meanwhile, it is possible to explain some discrepancy between the authors at the description of karyotypes of the representatives of one species (discrepancy in diploid numbers, description of morphology of macrochromosomes) not only methodical or different interpretation of morphological types of chromosomes, but also interpopulation distinctions. It is not known, whether have different subspecies of penguins karyologycal differences (for example by quantityof microchromosomes).
In addition to karyologycal analysis for obtaining of the information about interpopulation differences it is possible to apply analysis of cytogenetic manifestations of genome instability. In this case we can obtain the data about fastness of a genome of representatives of various birds populations to conditions of habitation.
The cytogenetic approaches can allow to obtain highly informative data concerning differences between various systematical groups of birds. However, significant elaboration of the approaches is required, especial for penguins.


An authors express one's thanks to the INTAS for support of this research (INTAS project 01-0517).



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